pgrna humanized vector Search Results


pgrna humanized vector  (Addgene inc)
94
Addgene inc pgrna humanized vector
Pgrna Humanized Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgrna+humanized+vector/bio_rxiv__2021__07__13__452118-296-6-9?v=Addgene+inc
Average 94 stars, based on 1 article reviews
pgrna humanized vector - by Bioz Stars, 2026-08
94/100 stars
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a pgrna vector  (Addgene inc)
96
Addgene inc a pgrna vector
( A ) Schematic representation of expression vectors, <t>pgRNA</t> vector, pCAG-NFL- hCas9 , pCAG/ EndoGalC -29, and pPGK-pac. CAG, cytomegalovirus enhancer + chicken β-actin promoter; hCas9, humanized Cas9 gene; p(A), poly(A) tail; EndoGalC , Clostridium perfringens -derived endo-β-galactosidase gene; Kan r , kanamycin resistance gene; Amp r , ampicillin resistance <t>gene;</t> <t>U6,</t> human U6 promoter; PGKp, mouse phosphoglycerol kinase promoter; pac , puromycin N -acetyltransferase gene. ( B ) Flowchart of the experiments used to test the feasibility of a new system for the enrichment of genome-edited cells. Cells are co-transfected with three vectors, namely pgRNA, pCAG-NFL-hCas9, and pCAG/ EndoGalC -29, as the experimental group. Three days after transfection, cells are treated with IB4SAP for a short period, prior to cultivation in a normal medium. For control group-1, cells are transfected with pgRNA and pCAG-NFL- hCas9 . Three days after transfection, they are split to one tenth of the total amount, prior to cultivation in a normal medium. For control group-2, cells are transfected with pgRNA, pCAG-NFL- hCas9 , and pPGK- pac . Three days after transfection, they are split to one sixth of the total amount, prior to cultivation in a medium containing puromycin for 2 days. The emerging colonies are propagated for molecular biological and cytochemical analyses.
A Pgrna Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgrna+humanized+vector/pmc05979347-120-11-14?v=Addgene+inc
Average 96 stars, based on 1 article reviews
a pgrna vector - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier
phtn ghalotag cmv neo halotag vector  (OriGene)
93
OriGene phtn ghalotag cmv neo halotag vector
( A ) Schematic representation of expression vectors, <t>pgRNA</t> vector, pCAG-NFL- hCas9 , pCAG/ EndoGalC -29, and pPGK-pac. CAG, cytomegalovirus enhancer + chicken β-actin promoter; hCas9, humanized Cas9 gene; p(A), poly(A) tail; EndoGalC , Clostridium perfringens -derived endo-β-galactosidase gene; Kan r , kanamycin resistance gene; Amp r , ampicillin resistance <t>gene;</t> <t>U6,</t> human U6 promoter; PGKp, mouse phosphoglycerol kinase promoter; pac , puromycin N -acetyltransferase gene. ( B ) Flowchart of the experiments used to test the feasibility of a new system for the enrichment of genome-edited cells. Cells are co-transfected with three vectors, namely pgRNA, pCAG-NFL-hCas9, and pCAG/ EndoGalC -29, as the experimental group. Three days after transfection, cells are treated with IB4SAP for a short period, prior to cultivation in a normal medium. For control group-1, cells are transfected with pgRNA and pCAG-NFL- hCas9 . Three days after transfection, they are split to one tenth of the total amount, prior to cultivation in a normal medium. For control group-2, cells are transfected with pgRNA, pCAG-NFL- hCas9 , and pPGK- pac . Three days after transfection, they are split to one sixth of the total amount, prior to cultivation in a medium containing puromycin for 2 days. The emerging colonies are propagated for molecular biological and cytochemical analyses.
Phtn Ghalotag Cmv Neo Halotag Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgrna+humanized+vector/pm27307215-35-10-17?v=OriGene
Average 93 stars, based on 1 article reviews
phtn ghalotag cmv neo halotag vector - by Bioz Stars, 2026-08
93/100 stars
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paper pgrna empty addgene  (Addgene inc)
96
Addgene inc paper pgrna empty addgene
KEY RESOURCES TABLE
Paper Pgrna Empty Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgrna+humanized+vector/pmc05823762-692-6-9?v=Addgene+inc
Average 96 stars, based on 1 article reviews
paper pgrna empty addgene - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier

Image Search Results


( A ) Schematic representation of expression vectors, pgRNA vector, pCAG-NFL- hCas9 , pCAG/ EndoGalC -29, and pPGK-pac. CAG, cytomegalovirus enhancer + chicken β-actin promoter; hCas9, humanized Cas9 gene; p(A), poly(A) tail; EndoGalC , Clostridium perfringens -derived endo-β-galactosidase gene; Kan r , kanamycin resistance gene; Amp r , ampicillin resistance gene; U6, human U6 promoter; PGKp, mouse phosphoglycerol kinase promoter; pac , puromycin N -acetyltransferase gene. ( B ) Flowchart of the experiments used to test the feasibility of a new system for the enrichment of genome-edited cells. Cells are co-transfected with three vectors, namely pgRNA, pCAG-NFL-hCas9, and pCAG/ EndoGalC -29, as the experimental group. Three days after transfection, cells are treated with IB4SAP for a short period, prior to cultivation in a normal medium. For control group-1, cells are transfected with pgRNA and pCAG-NFL- hCas9 . Three days after transfection, they are split to one tenth of the total amount, prior to cultivation in a normal medium. For control group-2, cells are transfected with pgRNA, pCAG-NFL- hCas9 , and pPGK- pac . Three days after transfection, they are split to one sixth of the total amount, prior to cultivation in a medium containing puromycin for 2 days. The emerging colonies are propagated for molecular biological and cytochemical analyses.

Journal: International Journal of Molecular Sciences

Article Title: The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones

doi: 10.3390/ijms19041075

Figure Lengend Snippet: ( A ) Schematic representation of expression vectors, pgRNA vector, pCAG-NFL- hCas9 , pCAG/ EndoGalC -29, and pPGK-pac. CAG, cytomegalovirus enhancer + chicken β-actin promoter; hCas9, humanized Cas9 gene; p(A), poly(A) tail; EndoGalC , Clostridium perfringens -derived endo-β-galactosidase gene; Kan r , kanamycin resistance gene; Amp r , ampicillin resistance gene; U6, human U6 promoter; PGKp, mouse phosphoglycerol kinase promoter; pac , puromycin N -acetyltransferase gene. ( B ) Flowchart of the experiments used to test the feasibility of a new system for the enrichment of genome-edited cells. Cells are co-transfected with three vectors, namely pgRNA, pCAG-NFL-hCas9, and pCAG/ EndoGalC -29, as the experimental group. Three days after transfection, cells are treated with IB4SAP for a short period, prior to cultivation in a normal medium. For control group-1, cells are transfected with pgRNA and pCAG-NFL- hCas9 . Three days after transfection, they are split to one tenth of the total amount, prior to cultivation in a normal medium. For control group-2, cells are transfected with pgRNA, pCAG-NFL- hCas9 , and pPGK- pac . Three days after transfection, they are split to one sixth of the total amount, prior to cultivation in a medium containing puromycin for 2 days. The emerging colonies are propagated for molecular biological and cytochemical analyses.

Article Snippet: The plasmid structure used in this study is schematically illustrated in A. pgRNA vector (Addgene, Cambridge, MA, USA) has a human U6 promoter to drive transcription of the downstream gRNA.

Techniques: Expressing, Plasmid Preparation, Derivative Assay, Transfection

KEY RESOURCES TABLE

Journal: Neuron

Article Title: Rbfox splicing factors promote neuronal maturation and axon initial segment assembly

doi: 10.1016/j.neuron.2018.01.020

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: NA NA Recombinant DNA pCAGGS-Cas9-mCherry This paper pgRNA empty Addgene #41824 pgRNA Rbfox1 KO This paper pgRNA Rbfox2 KO This paper pgRNA Rbfox3 KO This paper pgRNA1 AnkG del This paper pgRNA2 AnkG del This paper pgRNA1 AnkG ins This paper pgRNA2 AnkG ins This paper pCAGGS-3xFLAG-Rbfox1 This paper pCAGGS-3xFLAG-Rbfox2 This paper pCAGGS-3xFLAG-Rbfox3 This paper pCAGGS-3xFLAG-bIV spectrin (repeats 13–15) This paper pCAGGS-3xFLAG-bII spectrin (repeats 13–15) This paper pCAGGS-AnkG ZU5in-3xHA This paper pCAGGS-AnkG ZU5ex-3xHA This paper pCAGGS-AnkB ZU5ex-3xHA This paper Software and Algorithms AIS quantification algorithm (Matlab) ( Grubb and Burrone, 2010 ) (ais_z3.m) http://grubblab.org/resources/ Fiji ( Schindelin et al., 2012 ) https://fiji.sc/ RNA-seq analysis by Quantas ( Yan et al., 2015 ) http://zhanglab.c2b2.columbia.edu/index.php/Quantas CLIP data analysis by CTK ( Shah et al., 2017 ) http://zhanglab.c2b2.columbia.edu/index.php/CTK Open in a separate window KEY RESOURCES TABLE

Techniques: Produced, Recombinant, Software